Back to Search
Start Over
Sustained delivery of FGF-1 increases vascular density in comparison to bolus administration.
- Source :
-
Microvascular research [Microvasc Res] 2009 Sep; Vol. 78 (2), pp. 142-7. Date of Electronic Publication: 2009 Jun 23. - Publication Year :
- 2009
-
Abstract
- The use of growth factors for the therapeutic stimulation of neovascularization in regenerative medicine has been extensively investigated, but the inability to control their temporal delivery may limit clinical success. A strategy that delivers continuous therapeutic concentrations of growth factors may increase the protein's efficacy. The present study investigates the ability of sustained delivery of fibroblast growth factor-1 (FGF-1), to induce neovascularization in vivo. Alginate microbeads were synthesized to release active FGF-1 for three weeks. Microbeads loaded with FGF-1 (total amount 150 ng) were implanted into a surgically created omentum pouch in rats and were compared to control empty microbead implants and a single bolus injection of 150 ng of FGF-1 with empty microbead implant. Animals were sacrificed at either 3 or 6 weeks post implantation and omenta were analyzed for vascular density and mural cell interactions. Vascular area for bolus FGF-1 and FGF-1 loaded microbeads was higher than control at 3 weeks. At 6 weeks, vascular density in the group with FGF-1 loaded microbeads was significantly higher than the group with bolus administration of FGF-1, primarily due to an increase in the number of vessels less than 20 microm in diameter. Vascular density in omenta of the group receiving the bolus FGF-1 returned to control levels by 6 weeks. Staining for smooth muscle actin showed that 50% of vessels had associated mural cells. There was a trend of increased mural cell staining at 6 weeks for the FGF-1 loaded beads compared to bolus FGF-1 and control levels. Results in these studies suggest that sustained release of FGF-1 increases the duration of the vascular response in contrast to a bolus injection of FGF-1.
- Subjects :
- Actins metabolism
Alginates chemical synthesis
Alginates chemistry
Animals
Fibroblast Growth Factor 1 genetics
Glucuronic Acid chemical synthesis
Glucuronic Acid chemistry
Hexuronic Acids chemical synthesis
Hexuronic Acids chemistry
Immunohistochemistry
Microspheres
Omentum blood supply
Omentum metabolism
Platelet Endothelial Cell Adhesion Molecule-1 metabolism
Rats
Rats, Inbred Lew
Rats, Sprague-Dawley
Receptor, Fibroblast Growth Factor, Type 1 metabolism
Time Factors
Blood Vessels metabolism
Drug Delivery Systems
Fibroblast Growth Factor 1 administration & dosage
Fibroblast Growth Factor 1 metabolism
Neovascularization, Physiologic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9319
- Volume :
- 78
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microvascular research
- Publication Type :
- Academic Journal
- Accession number :
- 19555698
- Full Text :
- https://doi.org/10.1016/j.mvr.2009.06.006