Back to Search
Start Over
Motor-dependent microtubule disassembly driven by tubulin tyrosination.
- Source :
-
The Journal of cell biology [J Cell Biol] 2009 Jun 29; Vol. 185 (7), pp. 1159-66. - Publication Year :
- 2009
-
Abstract
- In cells, stable microtubules (MTs) are covalently modified by a carboxypeptidase, which removes the C-terminal Tyr residue of alpha-tubulin. The significance of this selective detyrosination of MTs is not understood. In this study, we report that tubulin detyrosination in fibroblasts inhibits MT disassembly. This inhibition is relieved by overexpression of the depolymerizing motor mitotic centromere-associated kinesin (MCAK). Conversely, suppression of MCAK expression prevents disassembly of normal tyrosinated MTs in fibroblasts. Detyrosination of MTs suppresses the activity of MCAK in vitro, apparently as the result of a decreased affinity of the adenosine diphosphate (ADP)-inorganic phosphate- and ADP-bound forms of MCAK for the MT lattice. Detyrosination also impairs MT disassembly in neurons and inhibits the activity of the neuronal depolymerizing motor KIF2A in vitro. These results indicate that MT depolymerizing motors are directly inhibited by the detyrosination of tubulin, resulting in the stabilization of cellular MTs. Detyrosination of transiently stabilized MTs may give rise to persistent subpopulations of disassembly-resistant polymers to sustain subcellular cytoskeletal differentiation.
- Subjects :
- Animals
Cell Shape
Cells, Cultured
Fibroblasts cytology
Fibroblasts metabolism
Hippocampus cytology
Kinesins genetics
Kinesins metabolism
Mice
Mice, Knockout
Molecular Motor Proteins genetics
Neurons cytology
Neurons metabolism
Nocodazole metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Tubulin Modulators metabolism
Microtubules metabolism
Molecular Motor Proteins metabolism
Spindle Apparatus metabolism
Tubulin metabolism
Tyrosine metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1540-8140
- Volume :
- 185
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 19564401
- Full Text :
- https://doi.org/10.1083/jcb.200902142