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Oxidative stress, DNA damage and antioxidant enzyme gene expression in the Pacific white shrimp, Litopenaeus vannamei when exposed to acute pH stress.
- Source :
-
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2009 Nov; Vol. 150 (4), pp. 428-35. Date of Electronic Publication: 2009 Jun 30. - Publication Year :
- 2009
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Abstract
- The ROS production, the percentage of dead and damaged haemocytes, the DNA Olive Tail Moment (OTM) value and the gene expression of manganese superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPx) and thioredoxin (TRx), were studied in the Pacific white shrimp, Litopenaeus vannamei, when exposed to acute pH stress. The increased ROS production in haemocytes and the increased OTM value in both the haemocytes and the hepatopancreas cells suggest that oxidative damage occurred in shrimp exposed to pH 5.6 and pH 9.3, with apoptosis, mainly being associated with excess Ca(2+)influx and changes in cell viability. Acid and alkaline pH-induced DNA damage was time dependent in the haemocytes and the hepatopancreas cells. The concentration of intracellular free calcium [Ca(2+)] (i) after different pH treatments increased significantly over time, reaching its highest concentration after 12 h, but decreasing gradually to normal levels after 24 h. The [Ca(2+)] (i) content in shrimp cells when exposed to pH 9.3 and pH 5.6 for 12 h had increased by 58%-81%, compared with exposure to pH 7.4 (control). In addition, the gene expression of cMnSOD, CAT, GPx and TRx in the hepatopancreas of L. vannamei was induced by acid and alkaline pH stress, although there were differences in the expression response with respect to the duration of induction and the different pH treatments (acid or alkaline). Our results show that acidic or alkaline-induced oxidative stress may cause DNA damage, and cooperatively activate expression of CAT, GPx and TRx mRNA. Calcium ions appear to be important in mediating shrimp responses to pH stress.
- Subjects :
- Animals
Apoptosis genetics
Calcium metabolism
Catalase metabolism
Cell Survival
Comet Assay
Gene Expression Regulation, Enzymologic
Glutathione Peroxidase metabolism
Hemocytes enzymology
Hemocytes metabolism
Hemolymph enzymology
Hemolymph metabolism
Hepatopancreas enzymology
Hepatopancreas metabolism
Hydrogen-Ion Concentration
Pacific Ocean
Penaeidae genetics
Penaeidae metabolism
Reactive Oxygen Species metabolism
Respiratory Burst
Superoxide Dismutase metabolism
Thioredoxins metabolism
Time Factors
Antioxidants metabolism
DNA Damage genetics
Oxidative Stress genetics
Penaeidae enzymology
Stress, Physiological
Subjects
Details
- Language :
- English
- ISSN :
- 1532-0456
- Volume :
- 150
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
- Publication Type :
- Academic Journal
- Accession number :
- 19573624
- Full Text :
- https://doi.org/10.1016/j.cbpc.2009.06.010