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Sex-reversed somatic cell cloning in the mouse.
- Source :
-
The Journal of reproduction and development [J Reprod Dev] 2009 Oct; Vol. 55 (5), pp. 566-9. Date of Electronic Publication: 2009 Jul 14. - Publication Year :
- 2009
-
Abstract
- Somatic cell nuclear transfer has many potential applications in the fields of basic and applied sciences. However, it has a disadvantage that can never be overcome technically-the inflexibility of the sex of the offspring. Here, we report an accidental birth of a female mouse following nuclear transfer using an immature Sertoli cell. We produced a batch of 27 clones in a nuclear transfer experiment using Sertoli cells collected from neonatal male mice. Among them, one pup was female. This "male-derived female" clone grew into a normal adult and produced offspring by natural mating with a littermate. Chromosomal analysis revealed that the female clone had a 39,X karyotype, indicating that the Y chromosome had been deleted in the donor cell or at some early step during nuclear transfer. This finding suggests the possibility of resuming sexual reproduction after a single male is cloned, which should be especially useful for reviving extinct or endangered species.
Details
- Language :
- English
- ISSN :
- 0916-8818
- Volume :
- 55
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 19602850
- Full Text :
- https://doi.org/10.1262/jrd.09-099e