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Integration of single and multicellular wound responses.
- Source :
-
Current biology : CB [Curr Biol] 2009 Aug 25; Vol. 19 (16), pp. 1389-95. Date of Electronic Publication: 2009 Jul 23. - Publication Year :
- 2009
-
Abstract
- Single cells and multicellular tissues rapidly heal wounds. These processes are considered distinct, but one mode of healing--Rho GTPase-dependent formation and closure of a purse string of actin filaments (F-actin) and myosin-2 around wounds--occurs in single cells and in epithelia. Here, we show that wounding of one cell in Xenopus embryos elicits Rho GTPase activation around the wound and at the nearest cell-cell junctions in the neighbor cells. F-actin and myosin-2 accumulate at the junctions and around the wound itself, and as the resultant actomyosin array closes over the wound site, junctional F-actin and myosin-2 become mechanically integrated with the actin and myosin-2 around the wound, forming a hybrid purse string. When cells are ablated rather than wounded, Rho GTPase activation and F-actin accumulation occur at cell-cell junctions surrounding the ablated cell, and the purse string closes the hole in the epithelium. Elevation of intracellular free calcium, an essential upstream signal for the single-cell wound response, also occurs at the cell-cell contacts and in neighbor cells. Thus, the single and multicellular purse string wound responses represent points on a signaling and mechanical continuum that are integrated by cell-cell junctions.
- Subjects :
- Animals
Blastomeres
Calcium Signaling
Embryo, Nonmammalian injuries
Embryo, Nonmammalian metabolism
Enzyme Activation
Wound Healing genetics
Xenopus laevis embryology
Actins physiology
Intercellular Junctions physiology
Myosin Type II physiology
Wound Healing physiology
rho GTP-Binding Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0445
- Volume :
- 19
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Current biology : CB
- Publication Type :
- Academic Journal
- Accession number :
- 19631537
- Full Text :
- https://doi.org/10.1016/j.cub.2009.06.044