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Salvianolic acid B-vitamin C synergy in cardiac differentiation from embryonic stem cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2009 Oct 02; Vol. 387 (4), pp. 723-8. Date of Electronic Publication: 2009 Jul 28. - Publication Year :
- 2009
-
Abstract
- Inefficient cardiomyocyte differentiation limits the therapeutic use of embryonic stem (ES) cell-derived cardiomyocytes. While large collections of proprietary chemicals had been screened to improve ES cell differentiation into cardiomyocytes, the natural product library remained unexplored. Using a mouse ES cell line transfected with a cardiomyocyte-specific alpha-myosin heavy chain promoter-driven enhanced green fluorescent protein (EGFP) reporter, we screened 24 natural products with known cardioprotective actions. Salvianolic acid B (saB), while produced minimal effect on its own, concentration-dependently synergized with vitamin C in inducing cardiomyocyte differentiation, as demonstrated by an increase in EGFP(+) cells, beating area in embryoid bodies, and expression of cardiomyocyte maturity markers. This synergy is specific to cardiomyocyte differentiation, and is involved with collagen synthesis. The present study demonstrates the saB-vitamin C synergy in inducing ES cell differentiation into matured and functional cardiomyocytes, and this may lead to a practicable cocktail approach to generate ES cell-derived cardiomyocytes for cardiac stem cell therapy.
- Subjects :
- Animals
Benzofurans isolation & purification
Cardiotonic Agents isolation & purification
Cell Line
Collagen biosynthesis
Drug Evaluation, Preclinical
Drug Synergism
Embryonic Stem Cells physiology
Mice
Ascorbic Acid pharmacology
Benzofurans pharmacology
Cardiotonic Agents pharmacology
Cell Differentiation drug effects
Embryonic Stem Cells drug effects
Myocytes, Cardiac physiology
Vitamins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 387
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 19643081
- Full Text :
- https://doi.org/10.1016/j.bbrc.2009.07.122