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Analysis of pesticide residues by fast gas chromatography in combination with negative chemical ionization mass spectrometry.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2009 Aug 28; Vol. 1216 (35), pp. 6326-34. Date of Electronic Publication: 2009 Jul 14. - Publication Year :
- 2009
-
Abstract
- A combination of fast GC with narrow-bore column and bench top quadrupole mass spectrometer (MS) detector in negative chemical ionization (NCI) mode (with methane as reagent gas) is set up and utilized for the ultratrace analysis of 25 selected pesticides. The observed pesticides, belonging to the endocrine disrupting chemicals (EDCs), were from different chemical classes. A comparative study with electron impact (EI) ionization was also carried out (both techniques in selected ion monitoring (SIM) mode). The programmed temperature vaporizer (PTV) injector in solvent vent mode and narrow-bore column (15mx0.15mm I.D.x0.15microm film of 5% diphenyl 95% dimethylsiloxane stationary phase) were used for effective and fast separation. Heptachlor (HPT) as internal standard (I.S.) was applied for the comparison of results obtained from absolute and normalized peak areas. Non-fatty food matrices were investigated. Fruit (apple - matrix-matched standards; orange, strawberry, plum - real samples) and vegetable (lettuce - real sample) extracts were prepared by a quick and effective QuEChERS sample preparation technique. Very good results were obtained for the characterization of fast GC-NCI-MS method analysing EDCs pesticides. Analyte response was linear from 0.01 to 150microgkg(-1) with the R(2) values in the range from 0.9936 to 1.0000 (calculated from absolute peak areas) and from 0.9956 to 1.0000 (calculated from peak areas normalized to HPT). Instrument limits of detection (LODs) and quantification (LOQs) were found at pgmL(-1) level and for the majority of analytes were up to three orders of magnitude lower for NCI compared to EI mode. In both ionization modes, repeatability of measurements expressed as relative standard deviation (RSDs) was less than 10% which is in very good agreement with the criterion of European Union.
- Subjects :
- Aminoimidazole Carboxamide analogs & derivatives
Aminoimidazole Carboxamide analysis
Aminoimidazole Carboxamide isolation & purification
Endocrine Disruptors analysis
Endocrine Disruptors isolation & purification
European Union
Fruit chemistry
Hydantoins analysis
Hydantoins isolation & purification
Malathion analysis
Malathion isolation & purification
Pesticide Residues isolation & purification
Pesticides isolation & purification
Vegetables chemistry
Food Analysis standards
Gas Chromatography-Mass Spectrometry methods
Pesticide Residues analysis
Pesticides analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1216
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 19643423
- Full Text :
- https://doi.org/10.1016/j.chroma.2009.07.013