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Efficacy of an indirect immunofluorescence test in the diagnosis of canine leishmaniosis.
- Source :
-
Veterinary journal (London, England : 1997) [Vet J] 2010 Oct; Vol. 186 (1), pp. 123-4. Date of Electronic Publication: 2009 Aug 07. - Publication Year :
- 2010
-
Abstract
- Of 146 dogs from a visceral leishmaniosis-endemic area that tested seronegative by indirect immunofluorescence (IIF) on blood samples collected on filter paper (IIFp), 51 (34.9%) and 10 (6.8%) tested positive by IIF on serum samples (IIFs) and enzyme immunoassay, respectively. Three samples (2.0%) tested positive by PCR. Leishmania chagasi was isolated from the skin of five (3.4%) dogs. Amastigote forms were identified in two of these five animals following histopathological and immunohistochemical examination. The findings highlight that detection methods such as IIFp can permit dogs infected with L. chagasi to remain undetected in endemic areas with attendant consequences for the epidemiology of infection both in the canine and human populations.<br /> (Copyright © 2009 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Dogs
Endemic Diseases prevention & control
Endemic Diseases veterinary
Fluorescent Antibody Technique, Indirect methods
Humans
Leishmania classification
Leishmaniasis, Cutaneous diagnosis
Polymerase Chain Reaction methods
Polymerase Chain Reaction veterinary
Sensitivity and Specificity
Dog Diseases diagnosis
Fluorescent Antibody Technique, Indirect veterinary
Leishmania isolation & purification
Leishmaniasis, Cutaneous veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2971
- Volume :
- 186
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Veterinary journal (London, England : 1997)
- Publication Type :
- Academic Journal
- Accession number :
- 19665398
- Full Text :
- https://doi.org/10.1016/j.tvjl.2009.06.030