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A liquid chromatography-electrospray ionization-tandem mass spectrometry method for quantitation of aripiprazole in human plasma.
- Source :
-
Journal of analytical toxicology [J Anal Toxicol] 2009 Jun; Vol. 33 (5), pp. 237-42. - Publication Year :
- 2009
-
Abstract
- A rugged liquid chromatographic-electrospray ionization-tandem mass spectrometric (LC-ESI-MS-MS) method was developed and validated for accurate monitoring of steady-state plasma aripiprazole. Haloperidol-d(4) was chosen as the internal standard. ESI of aripiprazole and haloperidol-d(4) yielded abundant MH(+) ions, m/z 448 and 379, respectively. These ions were collision-dissociated to respective product ions of m/z 285 and 168. Ion-suppression experiments with blank plasma extracts showed substantial depressions of the product ions at retention times between 0.5 to 2 min, prohibiting development of a high-throughput LC-MS-MS method. A steep-gradient elution LC permitted a robust LC-ESI-MS-MS method with a 12-min analysis time. Aripiprazole was quantified from 0.2-mL aliquots of human plasma with acceptable precision and accuracy down to a lower limit of quantitation of 2 ng/mL. Aripiprazole was stable in plasma samples stored at room temperature for 24 h or exposed to three freeze-thaw cycles and in processed extracts stored at -20 degrees C for six days or on the autosampler at 10 degrees C for four days. The method has been successfully used for determinations of steady-state concentrations of aripiprazole in human subjects given daily oral doses of 15 mg. All measured concentrations were well within the quantitative range of 2 to 400 ng/mL.
- Subjects :
- Administration, Oral
Adolescent
Adult
Antipsychotic Agents pharmacology
Aripiprazole
Chromatography, High Pressure Liquid
Haloperidol blood
Humans
Middle Aged
Piperazines pharmacology
Quinolones pharmacology
Reproducibility of Results
Young Adult
Antipsychotic Agents blood
Piperazines blood
Quinolones blood
Spectrometry, Mass, Electrospray Ionization methods
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 0146-4760
- Volume :
- 33
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of analytical toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 19671242
- Full Text :
- https://doi.org/10.1093/jat/33.5.237