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Time-dependent reduction of glutamine synthetase in retina of diabetic rats.
- Source :
-
Experimental eye research [Exp Eye Res] 2009 Dec; Vol. 89 (6), pp. 967-71. Date of Electronic Publication: 2009 Aug 20. - Publication Year :
- 2009
-
Abstract
- Diabetic Retinopathy (DR) is one of the most common complications of diabetes and a major cause of blindness worldwide. We studied the transcriptome of the diabetic retina using Series Analysis of Gene Expression (SAGE) technology and observed a 45.6% reduction in transcript levels of glutamine synthetase (GS) in streptozotocin-induced diabetic rats compared with normal rats. RT-PCR and colorimetric enzyme activity assays revealed significant differences in GS mRNA expression and enzyme activity as early as the first month of diabetes development, with a progressive decrease in GS mRNA level and enzyme activity over a 12-month period. Northern blot analysis indicated a linear correlation between the reduction in GS expression and the time course of diabetic retinopathy (r = 0.802, p < 0.0001), which was validated by real-time RT-PCR (r = 0.731, p < 0.001). Our results implicate GS as a possible biomarker for evaluating the severity of developed diabetic retinopathy over the time course of diabetes progression.
- Subjects :
- Animals
Biomarkers metabolism
Blotting, Northern
Diabetes Mellitus, Experimental genetics
Diabetic Retinopathy genetics
Disease Progression
Gene Expression
Gene Expression Profiling methods
Gene Library
Glutamate-Ammonia Ligase genetics
RNA, Messenger genetics
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction methods
Diabetes Mellitus, Experimental enzymology
Diabetic Retinopathy enzymology
Glutamate-Ammonia Ligase metabolism
Retina enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0007
- Volume :
- 89
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Experimental eye research
- Publication Type :
- Academic Journal
- Accession number :
- 19699197
- Full Text :
- https://doi.org/10.1016/j.exer.2009.08.006