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Prevascularization of cardiac patch on the omentum improves its therapeutic outcome.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2009 Sep 01; Vol. 106 (35), pp. 14990-5. Date of Electronic Publication: 2009 Aug 24. - Publication Year :
- 2009
-
Abstract
- The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.
- Subjects :
- Animals
Cells, Cultured
Electrocardiography
Graft Survival
Male
Microscopy, Electron, Scanning
Myocardial Infarction physiopathology
Neovascularization, Physiologic
Omentum cytology
Rats
Rats, Sprague-Dawley
Transplantation, Heterotopic
Treatment Outcome
Cell Culture Techniques methods
Cell Differentiation
Heart Transplantation methods
Myocardial Infarction surgery
Omentum blood supply
Omentum surgery
Tissue Engineering methods
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 106
- Issue :
- 35
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 19706385
- Full Text :
- https://doi.org/10.1073/pnas.0812242106