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Genetic detection of extended-spectrum beta-lactamase-containing Escherichia coli isolates and vancomycin-resistant enterococci in fecal samples of healthy children.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2009 Sep; Vol. 15 (3), pp. 211-6. - Publication Year :
- 2009
-
Abstract
- One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum beta-lactamases (ESBLs) containing Escherichia coli isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive E. coli isolates and the bla(CTX-M-1), bla(TEM-52), and bla(SHV-12) genes were identified in these isolates. The bla(TEM-52)-containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim-sulfamethoxazole, and chloramphenicol; sul1, sul3, and cmlA genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate). vanA-containing Escherichia faecium isolates were detected in 13 of the 112 fecal samples (11.6%), and vanC-1 isolates were found in 2 samples. A diversity of resistance genes [(tet(M), tet(L), erm(B), aph(3')-IIIa, ant(6)-Ia, catA, and vat(E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing E. coli and VRE isolates.
- Subjects :
- Adolescent
Anti-Bacterial Agents pharmacology
Child
Child, Preschool
Enterococcus drug effects
Enterococcus enzymology
Enterococcus genetics
Escherichia coli drug effects
Escherichia coli enzymology
Escherichia coli genetics
Genotype
Humans
Infant
Microbial Sensitivity Tests
Phenotype
beta-Lactamases metabolism
Disease Reservoirs
Enterococcus isolation & purification
Escherichia coli isolation & purification
Feces microbiology
Vancomycin Resistance
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 15
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 19728780
- Full Text :
- https://doi.org/10.1089/mdr.2009.0910