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Proteasomal activity is required to initiate and to sustain translational activation of messenger RNA encoding the stem-loop-binding protein during meiotic maturation in mice.
- Source :
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Biology of reproduction [Biol Reprod] 2010 Jan; Vol. 82 (1), pp. 123-31. Date of Electronic Publication: 2009 Sep 16. - Publication Year :
- 2010
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Abstract
- Developmentally regulated translation plays a key role in controlling gene expression during oogenesis. In particular, numerous mRNA species are translationally repressed in growing oocytes and become translationally activated during meiotic maturation. While many studies have focused on a U-rich sequence, termed the cytoplasmic polyadenylation element (CPE), located in the 3'-untranslated region (UTR) and the CPE-binding protein (CPEB) 1, multiple mechanisms likely contribute to translational control in oocytes. The stem-loop-binding protein (SLBP) is expressed in growing oocytes, where it is required for the accumulation of nonpolyadenylated histone mRNAs, and then accumulates substantially during meiotic maturation. We report that, in immature oocytes, Slbp mRNA carries a short poly(A) tail, and is weakly translated, and that a CPE-like sequence in the 3'-UTR is required to maintain this low activity. During maturation, Slbp mRNA becomes polyadenylated and translationally activated. Unexpectedly, proteasomal activity is required both to initiate and to sustain translational activation. This proteasomal activity is not required for the polyadenylation of Slbp mRNA during early maturation; however, it is required for a subsequent deadenylation of the mRNA that occurs during late maturation. Moreover, although CPEB1 is degraded during maturation, inhibiting its degradation by blocking mitogen-activated protein kinase 1/3 activity does not prevent the accumulation of SLBP, indicating that CPEB1 is not the protein whose degradation is required for translational activation of Slbp mRNA. These results identify a new role for proteasomal activity in initiating and sustaining translational activation during meiotic maturation.
- Subjects :
- 3' Untranslated Regions
Animals
Base Sequence
Female
Mice
Molecular Sequence Data
Poly A metabolism
Proteasome Endopeptidase Complex metabolism
RNA, Messenger metabolism
RNA-Binding Proteins
Transcription Factors metabolism
Gene Expression Regulation, Developmental
Meiosis
Nuclear Proteins metabolism
Oocytes metabolism
Oogenesis
Polyadenylation
mRNA Cleavage and Polyadenylation Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1529-7268
- Volume :
- 82
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 19759367
- Full Text :
- https://doi.org/10.1095/biolreprod.109.076588