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Bone sialoprotein, but not osteopontin, deficiency impairs the mineralization of regenerating bone during cortical defect healing.
- Source :
-
Bone [Bone] 2010 Feb; Vol. 46 (2), pp. 447-52. Date of Electronic Publication: 2009 Sep 15. - Publication Year :
- 2010
-
Abstract
- Bone healing is a complex multi-step process, which depends on the position and size of the lesion, and on the mechanical stability of the wounded area. To address more specifically the mechanisms involved in cortical bone healing, we created drill-hole defects in the cortex of mouse femur, a lesion that triggers intramembranous repair, and compared the roles of bone sialoprotein (BSP) and osteopontin (OPN), two proteins of the extracellular matrix, in the repair process. Bone regeneration was analyzed by ex vivo microcomputerized X-ray tomography and histomorphometry of bones of BSP-deficient, OPN-deficient and wild-type mice. In all mouse strains, the cortical gap was bridged with woven bone within 2 weeks and no mineralized tissue was observed in the marrow. Within 3 weeks, lamellar cortical bone filled the gap. The amount and degree of mineralization of the woven bone was not affected by OPN deficiency, but cortical bone healing was delayed in BSP-deficient mice due to delayed mineralization. Gene expression studies showed a higher amount of BSP transcripts in the repair bone of OPN-deficient mice, suggesting a possible compensation of OPN function by BSP in OPN-null mice. Our data suggest that BSP, but not OPN, plays a role in primary bone formation and mineralization of newly formed bone during the process of cortical bone healing.<br /> ((c) 2009 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Diaphyses diagnostic imaging
Diaphyses pathology
Diaphyses physiopathology
Femur diagnostic imaging
Femur physiopathology
Femur surgery
Gene Expression Regulation
Integrin-Binding Sialoprotein
Mice
Osteogenesis physiology
Osteopontin genetics
Osteopontin metabolism
Sialoglycoproteins genetics
Sialoglycoproteins metabolism
Wound Healing genetics
X-Ray Microtomography
Bone Regeneration physiology
Calcification, Physiologic physiology
Femur pathology
Osteopontin deficiency
Sialoglycoproteins deficiency
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2763
- Volume :
- 46
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Bone
- Publication Type :
- Academic Journal
- Accession number :
- 19761880
- Full Text :
- https://doi.org/10.1016/j.bone.2009.09.007