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Effects of intermittent high glucose on oxidative stress in endothelial cells.
- Source :
-
Acta diabetologica [Acta Diabetol] 2010 Dec; Vol. 47 Suppl 1, pp. 97-103. Date of Electronic Publication: 2009 Sep 11. - Publication Year :
- 2010
-
Abstract
- The objective of this study is to explore the mechanism of oxidative stress induced by intermittent high glucose in porcine iliac endothelial cells (PIECs). The PIECs were exposed to intermittent or constant high glucose for 3 or 6 days, and the mean fluorescent intensity (MFI) was measured via intracellular reactive oxygen species (ROS) captured by flow cytometry. The NADPH oxidase activity was measured by chemiluminescence with lucigenin. Intermittent high glucose induced a greater over-production of ROS than constant high glucose in PIECs; the NADPH oxidase activity was increased under both constant and intermittent high glucose conditions, being more marked in the latter (P < 0.05). In conclusion, intermittent high glucose induced more ROS in PIECs than constant high glucose, this effect seemed to be, at least in part related to the enhanced activation of NADPH oxidase. Glucose fluctuation may be involved in the development of vascular complications.
- Subjects :
- Animals
Cell Line
Endothelial Cells enzymology
Endothelium, Vascular cytology
Endothelium, Vascular enzymology
Glucose adverse effects
NADPH Oxidases metabolism
Reactive Oxygen Species metabolism
Swine
Endothelial Cells metabolism
Endothelium, Vascular metabolism
Glucose metabolism
Oxidative Stress
Subjects
Details
- Language :
- English
- ISSN :
- 1432-5233
- Volume :
- 47 Suppl 1
- Database :
- MEDLINE
- Journal :
- Acta diabetologica
- Publication Type :
- Academic Journal
- Accession number :
- 19763390
- Full Text :
- https://doi.org/10.1007/s00592-009-0140-5