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[Effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2009 Jun; Vol. 32 (6), pp. 923-5. - Publication Year :
- 2009
-
Abstract
- Objective: To investigate the effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells.<br />Methods: The proliferation inhibitory rate was evaluated by MTT assay. The telomerase activity was analyzed by TRAP-ELISA, and the expression of hTERT mRNA was determined by semi-quantitative RT-PCR.<br />Results: Matrine displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against SW1116 cells. Compared with control group, the telomerase activity and the expression of hTERT mRNA decreased significantly (P < 0.05 or P < 0.01) in matrine group.<br />Conclusion: Matrine can inhibit the telomerase activity by depressing the expression of hTERT in SW1116 cells and inhibiting cell proliferation.
- Subjects :
- Cell Line, Tumor
Colonic Neoplasms enzymology
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Enzyme-Linked Immunosorbent Assay methods
Gene Expression Regulation, Neoplastic drug effects
Humans
RNA, Messenger genetics
RNA, Messenger metabolism
Reverse Transcriptase Polymerase Chain Reaction
Telomerase genetics
Matrines
Alkaloids pharmacology
Antineoplastic Agents pharmacology
Cell Proliferation drug effects
Colonic Neoplasms pathology
Quinolizines pharmacology
Sophora chemistry
Telomerase metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 32
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 19764331