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Wound exudate as a proteomic window to reveal different mechanisms of tissue damage by snake venom toxins.
- Source :
-
Journal of proteome research [J Proteome Res] 2009 Nov; Vol. 8 (11), pp. 5120-31. - Publication Year :
- 2009
-
Abstract
- In light of the complexity of wound tissue, proteomic analysis may not clearly reveal the nature of the wound or the processes involved in healing. However, exudate associated with wounds may provide a "window" on cellular events leading to the development of the wound and/or its healing. In this investigation we performed proteomic analysis on wound exudates from muscular wounds in mice caused by two very different types of snake venom toxins: BaP1, a snake venom metalloproteinase and Mtx-I, a snake venom phospholipase A2. Proteomic analysis of the exudates associated with these wounds clearly differentiated them and offered new perspectives on functional mechanisms by which these toxins cause tissue damage. In the case of wounds caused by the metalloproteinase, there was evidence of degradation of nonfibrillar collagens whereas the phospholipase wound exudate was noted by the presence of fibrillar collagen type I, apolipoproteins A-I, A-IV, and E, and fibronectin. These results suggest that the hemorrhage caused by snake venom metalloproteinases may be associated with the degradation of specific extracellular matrix proteins which play a role in matrix/capillary stabilization and that release of apolipoproteins from their complexes may be involved with the dysfunctional hemostasis observed following snake envenoming.
- Subjects :
- Animals
Bothrops
Chromatography, Liquid methods
Extracellular Matrix Proteins metabolism
Keratins metabolism
Mass Spectrometry methods
Mice
Molecular Sequence Data
Skin cytology
Skin drug effects
Skin metabolism
Skin pathology
Snake Bites
Tandem Mass Spectrometry methods
Exudates and Transudates chemistry
Metalloendopeptidases pharmacology
Metalloendopeptidases toxicity
Muscle, Skeletal drug effects
Muscle, Skeletal metabolism
Muscle, Skeletal pathology
Phospholipases A2 pharmacology
Phospholipases A2 toxicity
Proteomics methods
Snake Venoms pharmacology
Snake Venoms toxicity
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1535-3907
- Volume :
- 8
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of proteome research
- Publication Type :
- Academic Journal
- Accession number :
- 19764775
- Full Text :
- https://doi.org/10.1021/pr900489m