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[Nuclear factor kappa B impairs insulin signaling pathway in skeletal muscle cells of rat with sepsis].
- Source :
-
Zhonghua wai ke za zhi [Chinese journal of surgery] [Zhonghua Wai Ke Za Zhi] 2009 Aug 15; Vol. 47 (16), pp. 1257-60. - Publication Year :
- 2009
-
Abstract
- Objective: To investigate the effects of nuclear factor kappa B (NF-kappaB) on insulin signaling in skeletal muscle cells of rat with sepsis.<br />Methods: SD rats were randomly divided into two groups: control group and sepsis group.Sepsis model was reproduced by cecal ligation and puncture in sepsis group. At 8, 16, 24, 48 and 72 h after operation, the gastrocnemius was harvested. Conventional HE staining was used to observe the morphology of skeletal muscle cells. IRS-1 protein and tyrosine phosphorylation of IRS-1 and Ser(307) phosphorylation of IRS-1 were detected by Western Blotting and immuno-precipitation. Activities of NF-kappaB in skeletal muscle cells were detected by electrophoretic mobility shift assay.<br />Results: Tyrosine phosphorylation of IRS-1 in sepsis group was significantly lower than in control group (P < 0.01), while Ser(307) phosphorylation of IRS-1 in sepsis group was significantly higher than in control group (P < 0.01). In sepsis group, NF-kappaB activity in skeletal muscle cells was significantly higher than in control group (P < 0.01). There was significant negative correlation between activity of NF-kappaB and tyrosine phosphorylation of IRS-1 (r = 0.972, P < 0.01). There was significant positive correlation between activities of NF-kappaB and Ser(307) phosphorylation of IRS-1 (r = 0.969, P < 0.01).<br />Conclusions: There is no inflammatory cell infiltrate in skeletal muscle cells with sepsis. But the activity of NF-kappaB in skeletal muscle cells is obviously enhanced, and it is closely related with disorder of insulin signaling in skeletal muscle cells of rat with sepsis.
- Subjects :
- Animals
Disease Models, Animal
Insulin Receptor Substrate Proteins metabolism
Male
Muscle Fibers, Skeletal pathology
NF-kappa B metabolism
Phosphorylation
Random Allocation
Rats
Rats, Sprague-Dawley
Sepsis pathology
Signal Transduction
Insulin metabolism
Muscle Fibers, Skeletal metabolism
NF-kappa B physiology
Sepsis metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-5815
- Volume :
- 47
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Zhonghua wai ke za zhi [Chinese journal of surgery]
- Publication Type :
- Academic Journal
- Accession number :
- 19781177