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Modulation of immunoresponse in BALB/c mice by oral administration of Fag e 1-glucomannan conjugate.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2009 Oct 28; Vol. 57 (20), pp. 9787-92. - Publication Year :
- 2009
-
Abstract
- Maillard-type glycosylation was applied to preparation of hypoallergenic agents from a major buckwheat allergen, Fag e 1. Conjugation with arabinogalactan (AG), xyloglucan (XG), or yeast glucomannan (YGM) successfully decreased in vitro allergenicity of Fag e 1. Determination of IgE titer in the tested allergic mice revealed that YGM was the most effective for in vivo allergenicity of Fag e 1 among these water-soluble polysaccharides. Real-time PCR analysis using a set of primer for IL-4 (a typical Th2 cytokine) or IFN-gamma (a typical Th1 cytokine) showed that expressed mRNA for IL-4 in splenocytes drastically decreased with increasing with Fag e 1-YGM conjugate feeding. In addition, based on a flow-cytometric analysis of T cell subsets in the splenocytes, it was confirmed that the feeding led to an improvement of Th1/Th2 balance in the allergic mice where population of Th1 increased from 2.91% to 4.02%, while that of Th2 decreased from 3.75% to 2.72%. Furthermore, it was revealed that differentiation ratio of regulatory T cell (Treg) in the splenocytes increased from 14.5% to 18.7% by the oral administration. These results indicated that Fag e 1-YGM conjugate can be available for an immunomodulating agent for buckwheat allergy.
- Subjects :
- Administration, Oral
Allergens administration & dosage
Allergens chemistry
Animals
Antigens, Plant
Cytokines immunology
Disease Models, Animal
Fagopyrum chemistry
Fagopyrum immunology
Female
Humans
Mannans administration & dosage
Mannans chemistry
Mannans metabolism
Mice
Mice, Inbred BALB C
Saccharomyces cerevisiae chemistry
Saccharomyces cerevisiae metabolism
Allergens immunology
Food Hypersensitivity immunology
Mannans immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 57
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19795882
- Full Text :
- https://doi.org/10.1021/jf902490t