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Identification of potential DREB2C targets in Arabidopsis thaliana plants overexpressing DREB2C using proteomic analysis.

Authors :
Lee K
Han KS
Kwon YS
Lee JH
Kim SH
Chung WS
Kim Y
Chun SS
Kim HK
Bae DW
Source :
Molecules and cells [Mol Cells] 2009 Oct 31; Vol. 28 (4), pp. 383-8. Date of Electronic Publication: 2009 Oct 13.
Publication Year :
2009

Abstract

The dehydration responsive element binding protein 2C (DREB2C) is a dehydration responsive element/C-repeat (DRE/CRT)-motif binding transcription factor that induced by mild heat stress. Previous experiments established that overexpression of DREB2C cDNA driven by the cauliflower mosaic virus 35S promoter (35S:DREB2C) resulted in increased heat tolerance in Arabidopsis. We first analyzed the proteomic profiles in wild-type and 35S:DREB2C plants at a normal temperature (22 degrees C), but could not detect any differences between the proteomes of wild-type and 35S:DREB2C plants. The transcript level of DREB2C in 35S:DREB2C plants after treatment with mild heat stress was increased more than two times compared with expression in 35S:DREB2C plants under unstressed condition. A proteomic approach was used to decipher the molecular mechanisms underlying thermotolerance in 35S:DREB2C Arabidopsis plants. Eleven protein spots were identified as being differentially regulated in 35S:DREB2C plants. Moreover, in silico motif analysis showed that peptidyl-prolyl isomerase ROC4, glutathione transferase 8, pyridoxal biosynthesis protein PDX1, and elongation factor Tu contained one or more DRE/CRT motifs. To our knowledge, this study is the first to identify possible targets of DREB2C transcription factors at the protein level. The proteomic results were in agreement with transcriptional data.

Details

Language :
English
ISSN :
0219-1032
Volume :
28
Issue :
4
Database :
MEDLINE
Journal :
Molecules and cells
Publication Type :
Academic Journal
Accession number :
19830397
Full Text :
https://doi.org/10.1007/s10059-009-0154-4