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Immune responses of airway neutrophils are impaired in asthma.
- Source :
-
Experimental lung research [Exp Lung Res] 2009 Sep; Vol. 35 (7), pp. 554-69. - Publication Year :
- 2009
-
Abstract
- Neutrophilic inflammation is a key effector arm of the innate immune response. Neutrophils may contribute significantly to airway inflammation in certain asthma subtypes. The objective of this study is to investigate the innate immune responses of isolated airway and circulating neutrophils in asthma. Participants with asthma (n = 17) and healthy volunteers (n = 11) underwent induced sputum and blood collection. Neutrophils were isolated from dispersed selected sputum and blood granulocytes using magnetic cell separation. Neutrophils were cultured with or without lipopolysaccharide (100 ng/mL) for 24 hours. Innate immune mediators were measured by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR). Airway neutrophils from participants with asthma spontaneously released lower levels of interleukin (IL)-8, IL-1beta and tumor necrosis factor-alpha proteins, and had lower levels of cytokine gene expression compared to healthy controls. Toll-like receptor 4 (TLR4) gene expression was significantly decreased in airway neutrophils from participants with asthma compared to healthy volunteers. Resting and lipopolysaccharide (LPS)-stimulated circulating neutrophils had lower levels of TLR2 and IL-1beta gene expression in asthma, but were otherwise similar to healthy controls. No differences were seen for matrix metalloproteinase (MMP)-9 release in asthma. Innate immune responses of airway neutrophil cells are impaired in asthmatic subjects on prophylactic therapy and may impact on susceptibility to, and severity of, airway infections.
- Subjects :
- Adult
Aged
Asthma genetics
Asthma metabolism
Case-Control Studies
Cytokines biosynthesis
Cytokines genetics
Female
Gene Expression
Humans
Immunity, Innate
In Vitro Techniques
Inflammation Mediators metabolism
Interleukin-1beta biosynthesis
Interleukin-1beta genetics
Lipopolysaccharides pharmacology
Male
Middle Aged
Neutrophils drug effects
Neutrophils metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Sputum cytology
Sputum immunology
Toll-Like Receptor 2 genetics
Toll-Like Receptor 4 genetics
Asthma immunology
Neutrophils immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-0499
- Volume :
- 35
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Experimental lung research
- Publication Type :
- Academic Journal
- Accession number :
- 19842845
- Full Text :
- https://doi.org/10.1080/01902140902777490