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Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2010 Mar; Vol. 34 (3), pp. 286-92. Date of Electronic Publication: 2009 Oct 30. - Publication Year :
- 2010
-
Abstract
- The cysteine-rich strongylocins were the first antimicrobial peptides (AMPs) discovered from the sea urchin species, Strongylocentrotus droebachiensis. Homologous putative proteins (called SpStrongylocin) were found in the sister species, S. purpuratus. To demonstrate that they exhibit the same antibacterial activity as strongylocins, cDNAs encoding the 'mature' peptides (SpStrongylocins 1 and 2) were cloned into a direct expression system fusing a protease cleavage site and two purification tags to the recombinant peptide. Both recombinant fusion peptides were expressed in a soluble form in an Escherichia coli strain tolerant to toxic proteins. Enterokinase was used to remove the fusion tags and purified recombinant SpStrongylocins 1 and 2 showed antimicrobial activity against both Gram-negative and Gram-positive bacteria. The results of membrane integrity assays against cytoplasmic membranes of E. coli suggest that both recombinant SpStrongylocins 1 and 2 conduct their antibacterial activity by intracellular killing mechanisms because no increase in membrane permeability was detected.<br /> (Copyright 2009 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Antimicrobial Cationic Peptides genetics
Base Sequence
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Recombinant Proteins genetics
Recombinant Proteins metabolism
Antimicrobial Cationic Peptides metabolism
Antimicrobial Cationic Peptides pharmacology
Gram-Negative Bacteria drug effects
Gram-Positive Bacteria drug effects
Strongylocentrotus purpuratus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 34
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 19852980
- Full Text :
- https://doi.org/10.1016/j.dci.2009.10.006