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Signal transduction of the melatonin receptor MT1 is disrupted in breast cancer cells by electromagnetic fields.
- Source :
-
Bioelectromagnetics [Bioelectromagnetics] 2010 Apr; Vol. 31 (3), pp. 237-45. - Publication Year :
- 2010
-
Abstract
- The growth of estrogen-receptor positive breast cancer cells is inhibited by the pineal gland hormone, melatonin. Concern has been raised that power-line frequency and microwave electromagnetic fields (EMFs) could reduce the efficiency of melatonin on breast cancer cells. In this study we investigated the impact of EMFs on the signal transduction of the high-affinity receptor MT1 in parental MCF-7 cells and MCF-7 cells transfected with the MT1 gene. The binding of the cAMP-responsive element binding (CREB) protein to a promoter sequence of BRCA-1 after stimulation with melatonin was analyzed by a gel-shift assay and the expression of four estrogen-responsive genes was measured in sham-exposed breast cancer cells and cells exposed to a sinusoidal 50 Hz EMF of 1.2 microT for 48 h. In sham-exposed cells, binding of CREB to the promoter of BRCA-1 was increased by estradiol and subsequently diminished by treatment with melatonin. In cells exposed to 1.2 microT, 50 Hz EMF, binding of CREB was almost completely omitted. Expression of BRCA-1, p53, p21(WAF), and c-myc was increased by estradiol stimulation and subsequently decreased by melatonin treatment in both cell lines, except for p53 expression in the transfected cell line, thereby proving the antiestrogenic effect of melatonin at molecular level. In contrast, in breast cancer cells transfected with MT1 exposed to 1.2 microT of the 50 Hz EMF, the expression of p53 and c-myc increased significantly after melatonin treatment but for p21(WAF) the increase was not significant. These results convincingly prove the negative effect of EMF on the antiestrogenic effect of melatonin in breast cancer cells.<br /> ((c) 2009 Wiley-Liss, Inc.)
- Subjects :
- BRCA1 Protein genetics
BRCA1 Protein metabolism
Breast Neoplasms genetics
Cell Line, Tumor
Cyclic AMP Response Element-Binding Protein metabolism
Cyclin-Dependent Kinase Inhibitor p21 genetics
Cyclin-Dependent Kinase Inhibitor p21 metabolism
Estradiol metabolism
Female
Gene Expression Regulation physiology
Gene Expression Regulation radiation effects
Genes, p53
Humans
Melatonin metabolism
Periodicity
Promoter Regions, Genetic
Proto-Oncogene Proteins c-myc genetics
Proto-Oncogene Proteins c-myc metabolism
Receptor, Melatonin, MT1 genetics
Signal Transduction radiation effects
Time Factors
Transfection
Breast Neoplasms metabolism
Electromagnetic Fields
Receptor, Melatonin, MT1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-186X
- Volume :
- 31
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Bioelectromagnetics
- Publication Type :
- Academic Journal
- Accession number :
- 19882681
- Full Text :
- https://doi.org/10.1002/bem.20554