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The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli.

The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli.

Authors :
Petty NK
Bulgin R
Crepin VF
Cerdeño-Tárraga AM
Schroeder GN
Quail MA
Lennard N
Corton C
Barron A
Clark L
Toribio AL
Parkhill J
Dougan G
Frankel G
Thomson NR
Source :
Journal of bacteriology [J Bacteriol] 2010 Jan; Vol. 192 (2), pp. 525-38. Date of Electronic Publication: 2009 Nov 06.
Publication Year :
2010

Abstract

Citrobacter rodentium (formally Citrobacter freundii biotype 4280) is a highly infectious pathogen that causes colitis and transmissible colonic hyperplasia in mice. In common with enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC, respectively), C. rodentium exploits a type III secretion system (T3SS) to induce attaching and effacing (A/E) lesions that are essential for virulence. Here, we report the fully annotated genome sequence of the 5.3-Mb chromosome and four plasmids harbored by C. rodentium strain ICC168. The genome sequence revealed key information about the phylogeny of C. rodentium and identified 1,585 C. rodentium-specific (without orthologues in EPEC or EHEC) coding sequences, 10 prophage-like regions, and 17 genomic islands, including the locus for enterocyte effacement (LEE) region, which encodes a T3SS and effector proteins. Among the 29 T3SS effectors found in C. rodentium are all 22 of the core effectors of EPEC strain E2348/69. In addition, we identified a novel C. rodentium effector, named EspS. C. rodentium harbors two type VI secretion systems (T6SS) (CTS1 and CTS2), while EHEC contains only one T6SS (EHS). Our analysis suggests that C. rodentium and EPEC/EHEC have converged on a common host infection strategy through access to a common pool of mobile DNA and that C. rodentium has lost gene functions associated with a previous pathogenic niche.

Details

Language :
English
ISSN :
1098-5530
Volume :
192
Issue :
2
Database :
MEDLINE
Journal :
Journal of bacteriology
Publication Type :
Academic Journal
Accession number :
19897651
Full Text :
https://doi.org/10.1128/JB.01144-09