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Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins.
- Source :
-
Acta crystallographica. Section D, Biological crystallography [Acta Crystallogr D Biol Crystallogr] 2009 Nov; Vol. 65 (Pt 11), pp. 1232-5. Date of Electronic Publication: 2009 Oct 22. - Publication Year :
- 2009
-
Abstract
- Crystallization of phytochromes and other photochromic proteins is hampered by the conformational changes that they undergo on exposure to light. As a canonical phytochrome, cyanobacterial Cph1 switches between two stable states upon absorption of red/far-red light. Consequently, it is mandatory to work in darkness from protein purification to crystal cryoprotection in order to ensure complete occupancy of one state or the other. With the simple and inexpensive methods that have been developed, phytochromes and other photochromic molecules can effectively be handled and crystallized, as has been demonstrated by the solution of the three-dimensional structure of the Cph1 sensory module.
Details
- Language :
- English
- ISSN :
- 1399-0047
- Volume :
- 65
- Issue :
- Pt 11
- Database :
- MEDLINE
- Journal :
- Acta crystallographica. Section D, Biological crystallography
- Publication Type :
- Academic Journal
- Accession number :
- 19923720
- Full Text :
- https://doi.org/10.1107/S0907444909034106