Back to Search
Start Over
[Effect of 131I-labeled recombinant human tumor necrosis factor-alpha on the nude mice bearing human hepatoma].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2009 Sep; Vol. 40 (5), pp. 787-92. - Publication Year :
- 2009
-
Abstract
- Objective: To test the effect of recombinant human tumor necrosis factor-alpha (rhTNF-alpha) labeled with 131I on tumor growth in the nude mice bearing human hepatoma.<br />Methods: The biological activity of 131I-rhTNF-alpha was tested through in vitro combination of the radioactive drug and SMMC-7721 cells. The BALB/c-nu/ nu mice model bearing human hepatoma were established by injecting single cell suspension of SMMC-7721 cells. When the tumor was 1 cm in diameter, the therapeutic experiment was carried out. Twenty-four nude mice bearing human hepatoma were divided into six groups according the area of tumor, weight and sex of the nude mice. After i. v administration of 131I-rhTNF-alpha, the body biodistribution of 131I-rhTNF-alpha was assessed during a short-term (30 min, 1 h, 6 h) and a long-term (12 h, 24 h, 48 h) period. The biological activity and tumor accumulation of 131I-rhTNF-alpha were investigated. In the end of the experiment, histopathologic examinations of tumor samples were undertaken.<br />Results: The combination of 131I-rhTNF-alpha with the human hepatoma SMMC-7721 cells existed dose dependence and saturability. The 131I-rhTNF-alpha had satisfactory immunoreactivity. The 131I-rhTNF-alpha accumulated in tumor tissues well and kept stable for several days. The tumor tissues accumulated the highest radioactivity at 6 h, and still maintained 67.5% of that radioactivity at 48 h. The 131I-rhTNF-alpha administrated either intravenously or intratumorally could inhibit tumor growth. The 131I-rhTNF-alpha had similar radioactivity as the positive control (P>0.05), but greater radioactivity than the control groups including 131I, rhTNF-alpha and the negative groups (P<0.05).<br />Conclusion: The radioactive compound of 131I-rhTNF-alpha can inhibit and kill tumor cells, which demonstrates its potential for treating hepatocarcinoma.
- Subjects :
- Animals
Carcinoma, Hepatocellular drug therapy
Carcinoma, Hepatocellular radiotherapy
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Iodine Radioisotopes therapeutic use
Liver Neoplasms drug therapy
Liver Neoplasms radiotherapy
Recombinant Proteins therapeutic use
Tumor Necrosis Factor-alpha therapeutic use
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 40
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 19950584