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The role of immunohistochemistry in discriminating primary from secondary extramammary Paget disease.
- Source :
-
The American Journal of dermatopathology [Am J Dermatopathol] 2010 Apr; Vol. 32 (2), pp. 137-43. - Publication Year :
- 2010
-
Abstract
- Background: Extramammary Paget disease (EMPD) is categorized into 2 groups: primary EMPD or EMPD secondary to underlying malignancy. Primary EMPD has a better prognosis, and the ability to distinguish between the 2 subsets has clinical relevance. Recent studies have suggested that immunostains, including cytokeratin (CK) 7, CK20, and BRST-2, distinguish between the 2 groups. We analyzed a large series of EMPD with an expanded immunohistochemical panel to assess its value in distinguishing primary from secondary disease.<br />Design: Formalin-fixed, paraffin-embedded sections of 98 EMPD specimens from 61 patients (45 primary and 16 secondary) were immunostained with CK7, CK20, HER-2/neu, BRST-2, CDX2, androgen receptor (AR), and cyclin D1. The study included 44 women and 17 men (median age: 73 years). Median follow-up time was 47 months.<br />Results: All EMPD specimens were vibrantly positive for CK7. The frequency of positivity for all EMPD samples was CK20 (28%), BRST-2 (40%), HER-2/neu (64%), CDX2 (10%), AR (16%), and cyclin D1 (76%). For primary EMPD, the frequency of positivity was CK20 (22%), BRST-2 (48%), HER-2/neu (65%), CDX2 (2%), AR (21%), and cyclin D1 (84%). For secondary EMPD, the frequency of positivity was CK20 (50%), BRST-2 (25%), HER-2/neu (60%), CDX2 (33%), AR (0%), and cyclin D1 (53%). Notably, all 6 of 7 cases of EMPD secondary to an anorectal adenocarcinoma tested were HER-2/neu negative and 5 of those 6 cases (80%) were CDX2 positive.<br />Conclusions: The role of CK7, CK20, and BRST-2 in distinguishing primary and secondary EMPD is limited because CK20 and BRST-2 were positive in large subsets of both groups. An expanded immunohistochemical panel, including HER-2/neu and CDX2, may be useful in discriminating primary EMPD from EMPD secondary to anorectal adenocarcinoma but fails to distinguish primary EMPD from EMPD secondary to urothelial or prostatic malignancy. The consistent overexpression of HER-2/neu in primary EMPD suggests a role for trastuzumab therapy in patients with recurrent disease.
- Subjects :
- Adenocarcinoma metabolism
Aged
Aged, 80 and over
CDX2 Transcription Factor
Carrier Proteins metabolism
Diagnosis, Differential
Female
Glycoproteins metabolism
Homeodomain Proteins metabolism
Humans
Keratin-20 metabolism
Keratin-7 metabolism
Male
Membrane Transport Proteins
Middle Aged
Paget Disease, Extramammary metabolism
Prostatic Neoplasms pathology
Receptor, ErbB-2 metabolism
Rectal Neoplasms pathology
Retrospective Studies
Skin Neoplasms metabolism
Urologic Neoplasms pathology
Adenocarcinoma diagnosis
Adenocarcinoma secondary
Immunohistochemistry methods
Paget Disease, Extramammary diagnosis
Paget Disease, Extramammary secondary
Skin Neoplasms diagnosis
Skin Neoplasms secondary
Subjects
Details
- Language :
- English
- ISSN :
- 1533-0311
- Volume :
- 32
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The American Journal of dermatopathology
- Publication Type :
- Academic Journal
- Accession number :
- 20051815
- Full Text :
- https://doi.org/10.1097/DAD.0b013e3181b71481