Purification and biochemical characterization of recombinant rice UDP-arabinopyranose mutase generated in insect cells.

Authors :: Konishi T
Miyazaki Y
Yamakawa S
Iwai H
Satoh S
Ishii T
Source :: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2010; Vol. 74 (1), pp. 191-4. Date of Electronic Publication: 2010 Jan 07.
Publication Year :: 2010
Abstract: Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.

Subjects :: Animals
Electrophoresis, Polyacrylamide Gel
Intramolecular Transferases genetics
Intramolecular Transferases metabolism
Kinetics
Recombinant Proteins genetics
Recombinant Proteins metabolism
Spodoptera cytology
Intramolecular Transferases biosynthesis
Intramolecular Transferases isolation & purification
Oryza enzymology
Recombinant Proteins biosynthesis
Recombinant Proteins isolation & purification
Spodoptera genetics
Uridine Diphosphate Sugars metabolism

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