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Role of ubiquitination in the DNA damage response: proteomic analysis to identify new DNA-damage-induced ubiquitinated proteins.
- Source :
-
Biochemical Society transactions [Biochem Soc Trans] 2010 Feb; Vol. 38 (Pt 1), pp. 87-91. - Publication Year :
- 2010
-
Abstract
- The DDR (DNA damage response) is a signalling transduction cascade utilizing many forms of post-translation modification of proteins, including phosphorylation and ubiquitination. The well-known function of ubiquitination is to target proteins for proteasomal degradation; however, it is also involved in the regulation of protein function. The present review describes how ubiquitination regulates the function of certain proteins involved in DDR, in particular FANCD2 (Fanconi's anaemia complementation group D2) and PCNA (proliferating-cell nuclear antigen). Also, the proteomic methods currently used to identify new ubiquitinated proteins in response to DNA damage, including the advantages of using the UBD (ubiquitin-binding domain) beads to purify the ubiquitinated proteins, are considered.
- Subjects :
- Animals
Carrier Proteins metabolism
Cell Cycle Proteins metabolism
Endodeoxyribonucleases
Fanconi Anemia Complementation Group D2 Protein genetics
Fanconi Anemia Complementation Group D2 Protein metabolism
Humans
Nuclear Proteins metabolism
Proliferating Cell Nuclear Antigen genetics
Proliferating Cell Nuclear Antigen metabolism
Protein Processing, Post-Translational
Protein Structure, Tertiary
Signal Transduction physiology
Ubiquitin chemistry
Ubiquitin metabolism
Ubiquitination
DNA Damage
Proteome analysis
Ubiquitinated Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1470-8752
- Volume :
- 38
- Issue :
- Pt 1
- Database :
- MEDLINE
- Journal :
- Biochemical Society transactions
- Publication Type :
- Academic Journal
- Accession number :
- 20074041
- Full Text :
- https://doi.org/10.1042/BST0380087