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The expression of SIAH1 is downregulated and associated with Bim and apoptosis in human breast cancer tissues and cells.
- Source :
-
Molecular carcinogenesis [Mol Carcinog] 2010 May; Vol. 49 (5), pp. 440-9. - Publication Year :
- 2010
-
Abstract
- Seven in absentia homolog1 (SIAH1) was reported as a tumor suppressor and played an important role in regulating cell apoptosis. However, its effects on the breast carcinogenesis remain unclear. In this study, our aims were to examine the relationship between SIAH1 and Bcl-2-interacting mediator of cell death (Bim) and to explore the effects of SIAH1 on the breast carcinogenesis. Immunohistochemical analysis in 231 cases of breast tissues showed that the expression of SIAH1 and Bim were significantly decreased in the breast carcinogenesis. Moreover, SIAH1 expression was significantly correlated with Bim. Both SIAH1 and Bim expression were significantly higher in well to moderately differentiated and in early-stage breast cancer. Reverse transcription (RT)-polymerase chain reaction (PCR) and Western blot analysis in paired breast cancer tissues and breast cell lines found that the expression of SIAH1 was lower in the breast cancer tissues and cell lines. SIAH1 inducing apoptosis of the breast cancer cells was dependent on Bim. However, SIAH1 inhibiting invasion of the breast cancer cells was independent of Bim. The increase of the function of SIAH1 to upregulate the expression of Bim may play an important role in the progression of breast cancer. Restoration of the function of SIAH1 may be a new therapeutic target of human breast cancer.<br /> ((c) 2010 Wiley-Liss, Inc.)
- Subjects :
- Base Sequence
Bcl-2-Like Protein 11
Breast Neoplasms pathology
Cell Line, Tumor
Cell Transformation, Neoplastic
DNA Primers
Female
Flow Cytometry
Humans
Immunohistochemistry
Middle Aged
Nuclear Proteins physiology
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Ubiquitin-Protein Ligases physiology
Apoptosis physiology
Apoptosis Regulatory Proteins metabolism
Breast Neoplasms metabolism
Down-Regulation
Membrane Proteins metabolism
Nuclear Proteins metabolism
Proto-Oncogene Proteins metabolism
Ubiquitin-Protein Ligases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-2744
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular carcinogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 20082325
- Full Text :
- https://doi.org/10.1002/mc.20615