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Long-term active immunization with a synthetic peptide corresponding to the second extracellular loop of β1-adrenoceptor induces both morphological and functional cardiomyopathic changes in rats.
- Source :
-
International journal of cardiology [Int J Cardiol] 2011 May 19; Vol. 149 (1), pp. 89-94. Date of Electronic Publication: 2010 Jan 22. - Publication Year :
- 2011
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Abstract
- Background: β1-adrenoceptors (β1-ARs) are the predominant receptors in regulating heart functions. β1-ARs contain 7-transmembrane domains (7TM), 3 extracellular loops and 3 intracellular loops. Among these loops, the second extracellular loop of β1-AR (β1-AR-ECII) plays an important role in the pathogenesis of heart failure.<br />Methods: (1) Select the sera-negative rats for antibodies against β1-AR-ECII. (2) Detect the level of antibodies against β1-AR-ECII in the process of active immunization with artificial synthetic peptides according to the sequence of human β1-AR-ECII. (3) Observe its long-term role on cardiac structure and function in vivo by immunizing rats. (4) Detect the changes of T lymphocytes subsets in the process of active immunization.<br />Results: The peptides induced the production of specific autoantibody against β1-AR-ECII. Furthermore, immunization with β1-AR-ECII peptide induced both morphological and functional alterations in the hearts of rats, which potentially results in heart failure. In addition, induction of the autoantibodies against β1-AR-ECII increased the CD4+/CD8+ ratio in the peripheral blood of rats.<br />Discussion: In this study, we determined the effect of anti-β1-AR-ECII autoantibody on morphological and functional cardiomyopathic alterations by immunization of rats with a synthetic peptide corresponding to the β1-AR-ECII for 18 months. These results provide further evidence that autoantibody against β1-AR-ECII is involved in the pathogenesis of heart failure. But the underlying mechanisms need further study.<br /> (Copyright © 2009 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Autoantibodies blood
Cardiomyopathies pathology
Disease Models, Animal
Extracellular Space
Heart Failure pathology
Heart Function Tests
Humans
Myocardium immunology
Myocardium pathology
Organ Size immunology
Peptide Fragments immunology
Protein Structure, Tertiary
Rats
Rats, Wistar
Receptors, Adrenergic, beta-1 chemistry
T-Lymphocyte Subsets immunology
Autoantibodies immunology
Cardiomyopathies immunology
Heart Failure immunology
Receptors, Adrenergic, beta-1 immunology
Vaccination methods
Subjects
Details
- Language :
- English
- ISSN :
- 1874-1754
- Volume :
- 149
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of cardiology
- Publication Type :
- Academic Journal
- Accession number :
- 20096470
- Full Text :
- https://doi.org/10.1016/j.ijcard.2009.12.023