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Homogentisic acid interference in routine urine creatinine determination.

Homogentisic acid interference in routine urine creatinine determination.

Authors :
Loken PR
Magera MJ
Introne W
Tortorelli S
Gavrilov D
Oglesbee D
Rinaldo P
Matern D
Raymond K
Source :
Molecular genetics and metabolism [Mol Genet Metab] 2010 May; Vol. 100 (1), pp. 103-4. Date of Electronic Publication: 2010 Jan 21.
Publication Year :
2010

Abstract

We report the artifactual elevation of homogentisic acid (HGA) in urine from alkaptonuric patients after replacing the creatinine method (Jaffe reaction) in our laboratory with an automated enzymatic method. Samples with elevated HGA by GC-MS had lower creatinine values as determined by the enzymatic method than by the Jaffe reaction. The low creatinine values were due to interference by HGA in the enzymatic method. The enzymatic method is unsuitable for creatinine determination in urine of patients with alkaptonuria.<br /> ((c) 2010 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1096-7206
Volume :
100
Issue :
1
Database :
MEDLINE
Journal :
Molecular genetics and metabolism
Publication Type :
Academic Journal
Accession number :
20138792
Full Text :
https://doi.org/10.1016/j.ymgme.2010.01.006