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Rapid automated blood sampling system for pharmacokinetics studies of cigarette smoking.
- Source :
-
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco [Nicotine Tob Res] 2010 Apr; Vol. 12 (4), pp. 319-25. Date of Electronic Publication: 2010 Feb 08. - Publication Year :
- 2010
-
Abstract
- Introduction: We developed an automated sampling system to allow multiple, discrete blood samples from a human participant to be collected rapidly and immediately following cigarette smoke exposure. We reported the details of the sampling system along with the results of a pilot study for evaluation of the system.<br />Methods: Components of the system include silastic tubing, solenoid pinch valves, a peristaltic pump, and a fraction collector. This system incorporates a smoking machine that allows precise delivery of cigarette smoke through a mouthpiece and intricate timing to correlate blood samples with smoke inhalation. All components are controlled via integration from a user interface and are fully customizable. We performed several tests to evaluate the equipment, including tubing dead volume, leakage tests, and sample reproducibility. We also performed a pilot study with 6 adult smokers, who received 6 controlled puffs of a research test cigarette. Each inhalation was followed by radial arterial blood collection (1 sample per second tapered to 1 sample every 4 s) for 1 min. Samples were evaluated for nicotine via liquid chromatography-tandem mass spectrometric methods.<br />Results: Sampling times and volumes were sufficient for nicotine analysis. No adverse effects were seen in the pilot study, and a 30-min washout period was deemed appropriate between puffs. A significant rise in plasma nicotine levels above baseline after inhalation of smoke was consistently detected in all participants.<br />Discussion: The unique advantage of this system is to allow rapid blood sampling after a puff of cigarette smoke, with the benefits of reproducibility, reduction in labor intensity, and high temporal resolution.
Details
- Language :
- English
- ISSN :
- 1469-994X
- Volume :
- 12
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
- Publication Type :
- Academic Journal
- Accession number :
- 20142418
- Full Text :
- https://doi.org/10.1093/ntr/ntp190