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A novel low-cost and easy to develop functionalization platform. Case study: aptamer-based detection of thrombin by surface plasmon resonance.
- Source :
-
Talanta [Talanta] 2010 Mar 15; Vol. 80 (5), pp. 2157-64. Date of Electronic Publication: 2009 Nov 12. - Publication Year :
- 2010
-
Abstract
- A novel low-cost platform to assess biomolecular interactions was investigated using surface plasmon resonance and an aptamer-based assay for thrombin detection. Gold SPR surface functionalized with a carboxylated cross-linked BSA film (cBSA) and commercially available carboxymethylated dextran chip (CM5) were used as immobilization platforms for the thrombin binding aptamer. The high end commercial instrument Biacore 3000 and a custom made FIA set-up involving TI Spreeta sensor (TSPR2K23) were used to assess different concentrations of thrombin within the range 0.1-150 nM both in buffer and in a complex matrix (plasma) using the obtained aptasensors. Based on data derived from both CM5 and cBSA platforms, the cBSA aptasensor exhibited good selectivity, stability and regeneration ability, both in buffer and in complex matrices (plasma), comparable with CM5.<br /> (Copyright (c) 2009 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Aptamers, Nucleotide chemistry
Biosensing Techniques economics
Biosensing Techniques methods
Cattle
Gold chemistry
Humans
Protein Binding
Sensitivity and Specificity
Serum Albumin, Bovine chemistry
Surface Plasmon Resonance economics
Surface Plasmon Resonance methods
Thrombin metabolism
Aptamers, Nucleotide metabolism
Biosensing Techniques instrumentation
Surface Plasmon Resonance instrumentation
Thrombin analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 80
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 20152466
- Full Text :
- https://doi.org/10.1016/j.talanta.2009.11.023