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Structure determination of the glycans of human-serum alpha 1-antichymotrypsin using 1H-NMR spectroscopy and deglycosylation by N-glycanase.
- Source :
-
European journal of biochemistry [Eur J Biochem] 1991 Apr 10; Vol. 197 (1), pp. 209-15. - Publication Year :
- 1991
-
Abstract
- alpha 1-Antichymotrypsin purified from normal human serum was separated by affinity chromatography into th ree microheterogeneous forms on a concanavalin-A-Sepharose column: a pass-through (peak 1), a retarded (peak 2) and a bound form (peaks 3 + 4). For each form the asparagine-linked carbohydrate chains were liberated as oligosaccharides by hydrazinolysis, submitted to reduction with NaBH4 after re-N-acetylation and further separated by affinity chromatography on a concanavalin-A-Sepharose column. The complete primary structure of the glycans was determined by high-resolution 1H-NMR spectroscopy. The results indicated the presence of disialyl diantennary and of trisialyl triantennary type glycanic structures, the latter being accompanied by traces of disialylated triantennary oligosaccharide. The N-glycanase was used for the deglycosylation of the unfractionated alpha 1-antichymotrypsin; the successive removal of the N-linked complex-type oligosaccharide side chains of alpha 1-antichymotrypsin was studied in the presence of detergents. From these experiments it is concluded that alpha 1-antichymotrypsin carries four oligosaccharide side chains. Moreover our results show that the peak 1 contains four triantennary glycans, the peak 2 three triantennary and one diantennary glycans while the bound peaks 3 + 4 possess, on average, about one triantennary and three diantennary glycans per molecule. Since we showed that the peak 4 contains mostly diantennary glycans, it can be deduced that in peak 3 there are molecules carrying two triantennary and two diantennary glycans and others carrying one triantennary and three diantennary glycans.
- Subjects :
- Amidohydrolases
Asparagine
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Affinity
Humans
Hydrogen
Immunoelectrophoresis, Two-Dimensional
Magnetic Resonance Spectroscopy methods
Molecular Sequence Data
Oligosaccharides isolation & purification
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Polysaccharides isolation & purification
alpha 1-Antichymotrypsin isolation & purification
alpha 1-Antitrypsin chemistry
alpha 1-Antitrypsin isolation & purification
Oligosaccharides chemistry
Polysaccharides chemistry
alpha 1-Antichymotrypsin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2956
- Volume :
- 197
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- European journal of biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2015821
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1991.tb15901.x