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Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis.
- Source :
-
World journal of gastroenterology [World J Gastroenterol] 2010 Feb 28; Vol. 16 (8), pp. 973-81. - Publication Year :
- 2010
-
Abstract
- Aim: To search for further immunodominant peptides of the pyruvate dehydrogenase complex E2-component (PDC-E2) recognized by antimitochondrial antibodies (AMA) in primary biliary cirrhosis (PBC).<br />Methods: Sera from 95 patients with PBC were tested by enzyme-linked immunosorbent assay against 33 synthetic overlapping peptides (25 amino acids; aa) covering the entire length of the E2-subunit of PDC-E2. Furthermore, the inner lipoyl peptide 167-184 was used in an unlipoylated and a lipoylated form as well as coupled to ovalbumin. Sera from 11 AMA negative/ANA positive PBC patients, 63 patients with other liver disorders and 22 healthy blood donors served as controls.<br />Results: Of the 95 PBC-sera, 74% reacted with the peptide 475-499 and 58% with the peptide 407-431 located within the catalytic domain of PDC-E2. Patients with other disorders or healthy controls were positive in only up to 18%. Antibodies to the unlipoylated and lipoylated peptide 167-184 within the inner lipoyl domain were found in only 5% and 11% of the PBC sera, respectively; using ovalbumin-coupled peptides, the incidence increased up to 57% (unlipoylated form).<br />Conclusion: Peptides within the catalytic site of PDC-E2 rather than the previously reported lipoyl binding peptide 167-184 may represent major immunodominant epitopes recognized by AMA in PBC.
- Subjects :
- Adult
Aged
Amino Acid Sequence
Autoantibodies blood
Dihydrolipoyllysine-Residue Acetyltransferase chemistry
Dihydrolipoyllysine-Residue Acetyltransferase genetics
Female
Humans
Immunodominant Epitopes immunology
Liver enzymology
Liver immunology
Liver pathology
Liver Cirrhosis, Biliary blood
Male
Middle Aged
Molecular Sequence Data
Peptides chemistry
Peptides genetics
Peptides immunology
Young Adult
Autoantibodies immunology
Catalytic Domain immunology
Dihydrolipoyllysine-Residue Acetyltransferase immunology
Epitopes immunology
Liver Cirrhosis, Biliary immunology
Mitochondria, Liver immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2219-2840
- Volume :
- 16
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- World journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 20180236
- Full Text :
- https://doi.org/10.3748/wjg.v16.i8.973