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Protein differential expression induced by endocrine disrupting compounds in a terrestrial isopod.
- Source :
-
Chemosphere [Chemosphere] 2010 Apr; Vol. 79 (5), pp. 570-6. Date of Electronic Publication: 2010 Mar 01. - Publication Year :
- 2010
-
Abstract
- Endocrine disrupting compounds (EDCs) have been studied due to their impact on human health and increasing awareness of their impact on wildlife species. Studies concerning the organ-specific molecular effects of EDC in invertebrates are important to understand the mechanisms of action of this class of toxicants but are scarce in the literature. We have used a dose/response approach to unravel the protein expression in different organs of isopods exposed to bisphenol A (BPA) and vinclozolin (Vz) and assess their potential use as surrogate species. Male isopods were exposed to a range of Vz or of BPA concentrations. After animal dissection, proteins were extracted from gut, hepatopancreas and testes. Protein profiles were analysed by electrophoresis and differentially expressed proteins were identified by MALDI mass spectrometry. EDCs affected proteins involved in the energy metabolism (arginine kinase), proteins of the heat shock protein family (Hsp70 and GRP78) and most likely microtubule dynamics (tubulin). Different proteins expressed at different concentrations in different organs are indicative of the organ-specific effects of BPA and Vz. Additionally, several proteins were up-regulated at lower but not higher BPA or Vz concentrations, bringing new data to the non-monotonic response curve controversy. Furthermore, our findings suggest that some common responses to EDCs in both vertebrates and invertebrates may exist.
- Subjects :
- Animals
Benzhydryl Compounds
Endoplasmic Reticulum Chaperone BiP
HSP70 Heat-Shock Proteins metabolism
Liver metabolism
Male
Oxazoles toxicity
Phenols toxicity
Proteome metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Testis metabolism
Endocrine Disruptors toxicity
Isopoda metabolism
Water Pollutants, Chemical toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1298
- Volume :
- 79
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Chemosphere
- Publication Type :
- Academic Journal
- Accession number :
- 20189627
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2010.01.055