Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Authors :: Tomás M
Doumith M
Warner M
Turton JF
Beceiro A
Bou G
Livermore DM
Woodford N
Source :: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2010 May; Vol. 54 (5), pp. 2219-24. Date of Electronic Publication: 2010 Mar 01.
Publication Year :: 2010
Abstract: Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.

Subjects :: Drug Resistance, Bacterial genetics
Electrophoresis, Gel, Pulsed-Field
Humans
Mutation
Pseudomonas Infections microbiology
Pseudomonas aeruginosa genetics
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation physiology
Bacterial Outer Membrane Proteins genetics
Bacterial Proteins genetics
Cystic Fibrosis complications
Drug Resistance, Multiple genetics
Membrane Transport Proteins genetics
Porins genetics
Pseudomonas Infections drug therapy
Pseudomonas aeruginosa drug effects
beta-Lactamases genetics

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