Identification of protein interaction regions of VINC/NEAT1/Men epsilon RNA.

Authors :: Murthy UM
Rangarajan PN
Source :: FEBS letters [FEBS Lett] 2010 Apr 16; Vol. 584 (8), pp. 1531-5. Date of Electronic Publication: 2010 Mar 06.
Publication Year :: 2010
Abstract: The virus inducible non-coding RNA (VINC) was detected initially in the brain of mice infected with Japanese encephalitis virus (JEV) and rabies virus. VINC is also known as NEAT1 or Men epsilon RNA. It is localized in the nuclear paraspeckles of several murine as well as human cell lines and is essential for paraspeckle formation. We demonstrate that VINC interacts with the paraspeckle protein, P54nrb through three different protein interaction regions (PIRs) one of which (PIR-1) is localized near the 5' end while the other two (PIR-2, PIR-3) are localized near the 3' region of VINC. Our studies suggest that VINC may interact with P54nrb through a novel mechanism which is different from that reported for protein coding RNAs.<br /> (Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)