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Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster.
- Source :
-
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2010 Jun; Vol. 37 (6), pp. 575-80. Date of Electronic Publication: 2010 Mar 06. - Publication Year :
- 2010
-
Abstract
- We have expressed the pqqABCDE gene cluster from Gluconobacter oxydans, which is involved in pyrroloquinoline quinone (PQQ) biosynthesis, in Escherichia coli, resulting in PQQ accumulation in the medium. Since the gene cluster does not include the tldD gene needed for PQQ production, this result suggests that the E. coli tldD gene, which shows high homology to the G. oxydans tldD gene, carries out that function. The synthesis of PQQ activated d-glucose dehydrogenase in E. coli and the growth of the recombinant was improved. In an attempt to increase the production of PQQ, which acts as a vitamin or growth factor, we transformed E. coli with various recombinant plasmids, resulting in the overproduction of the PQQ synthesis enzymes and, consequently, PQQ accumulation--up to 6 mM--in the medium. This yield is 21.5-fold higher than that obtained in previous studies.
- Subjects :
- Escherichia coli growth & development
Escherichia coli metabolism
Escherichia coli Proteins genetics
Genes, Bacterial
Genetic Vectors
Gluconobacter oxydans metabolism
Glucose 1-Dehydrogenase metabolism
Multigene Family
PQQ Cofactor genetics
Plasmids
Cloning, Molecular methods
Escherichia coli genetics
Gluconobacter oxydans genetics
PQQ Cofactor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5535
- Volume :
- 37
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of industrial microbiology & biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 20213113
- Full Text :
- https://doi.org/10.1007/s10295-010-0703-z