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End labeling of enzymatically decapped mRNA.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 1977 Dec; Vol. 4 (12), pp. 4165-74. - Publication Year :
- 1977
-
Abstract
- A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.
Details
- Language :
- English
- ISSN :
- 0305-1048
- Volume :
- 4
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 202926
- Full Text :
- https://doi.org/10.1093/nar/4.12.4165