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Endodontic photoactivated disinfection using a conventional light source: an in vitro and ex vivo study.
- Source :
-
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics [Oral Surg Oral Med Oral Pathol Oral Radiol Endod] 2010 Apr; Vol. 109 (4), pp. 634-41. - Publication Year :
- 2010
-
Abstract
- Objective: The antimicrobial effect of photoactivated disinfection (PAD) using toluidine blue and an LED lamp was tested on endodontic pathogens in planktonic suspension and after inoculation into extracted teeth. Irradiation time was limited to 30 seconds.<br />Study Design: The effect of PAD on planktonic suspensions of Escherichia coli, Candida albicans, Enterococcus faecalis, Fusobacterium nucleatum, and Streptococcus intermedius was analyzed using Poisson regression. Moreover, cultures of S. intermedius were inoculated into prepared root canals of extracted molars. The effect of PAD performed immediately after inoculation or after overnight bacterial incubation was determined by a 2-sample t test.<br />Results: Photoactivated disinfection yielded significant reductions (P < .001) in the viable counts of all organisms in planktonic suspension. The PAD treatment of S. intermedius in root canals yielded a mean log10 reduction of 2.60 (P < .001) immediately after inoculation and of 1.38 (P < .001) after overnight incubation.<br />Conclusion: Photoactivated disinfection using a conventional light source strongly reduces the number of viable endodontic pathogens in planktonic suspension and in root canals.<br /> (Copyright 2010 Mosby, Inc. All rights reserved.)
- Subjects :
- Bacteriological Techniques
Candida albicans drug effects
Candida albicans radiation effects
Colony Count, Microbial
Enterococcus faecalis drug effects
Enterococcus faecalis radiation effects
Escherichia coli drug effects
Escherichia coli radiation effects
Fusobacterium nucleatum drug effects
Fusobacterium nucleatum radiation effects
Humans
Materials Testing
Phototherapy instrumentation
Streptococcus intermedius drug effects
Streptococcus intermedius radiation effects
Time Factors
Bacteria drug effects
Dental Pulp Cavity microbiology
Disinfection methods
Photochemical Processes
Photosensitizing Agents therapeutic use
Phototherapy methods
Tolonium Chloride therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1528-395X
- Volume :
- 109
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
- Publication Type :
- Academic Journal
- Accession number :
- 20303060
- Full Text :
- https://doi.org/10.1016/j.tripleo.2009.12.027