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Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation.

Authors :
Pasapera AM
Schneider IC
Rericha E
Schlaepfer DD
Waterman CM
Source :
The Journal of cell biology [J Cell Biol] 2010 Mar 22; Vol. 188 (6), pp. 877-90.
Publication Year :
2010

Abstract

Focal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II-mediated cytoskeletal tension in a process known as FA maturation. To understand tension-mediated FA maturation, we sought to identify proteins that are recruited to FAs in a myosin II-dependent manner and to examine the mechanism for their myosin II-sensitive FA association. We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent. Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin. We show that phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. These results reveal an important role for paxillin in adhesion mechanosensing via myosin II-mediated FAK phosphorylation of paxillin that promotes vinculin FA recruitment to reinforce the cytoskeletal ECM linkage and drive FA maturation.

Details

Language :
English
ISSN :
1540-8140
Volume :
188
Issue :
6
Database :
MEDLINE
Journal :
The Journal of cell biology
Publication Type :
Academic Journal
Accession number :
20308429
Full Text :
https://doi.org/10.1083/jcb.200906012