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Curcumin dramatically enhances retinoic acid-induced superoxide generating activity via accumulation of p47-phox and p67-phox proteins in U937 cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2010 Apr 23; Vol. 395 (1), pp. 61-5. Date of Electronic Publication: 2010 Mar 25. - Publication Year :
- 2010
-
Abstract
- The membrane bound cytochrome b558 composed of large gp91-phox and small p22-phox subunits, and cytosolic proteins p40-, p47- and p67-phox are important components of superoxide (O(2)(-))-generating system in phagocytes and B lymphocytes. A lack of this system in phagocytes is known to cause serious life-threatening infections. Here, we describe that curcumin, a polyphenol responsible for the yellow color of curry spice turmeric, dramatically activates the O(2)(-)-generating system during retinoic acid (RA)-induced differentiation of human monoblastic leukemia U937 cells to macrophage-like cells. When U937 cells were cultured in the presence of RA and curcumin, the O(2)(-)-generating activity increased more than 4-fold compared with that in the absence of the latter. Semiquantitative RT-PCR showed that co-treatment with RA and curcumin slightly enhanced gene expressions of the five components compared with those of the RA-treatment only. On the other hand, immunoblot analysis revealed that co-treatment with RA and curcumin caused remarkable accumulation of protein levels of p47-phox (to 7-fold) and p67-phox (to 4-fold) compared with those of the RA-treatment alone. These results suggested that curcumin dramatically enhances RA-induced O(2)(-)-generating activity via accumulation of cytosolic p47-phox and p67-phox proteins in U937 cells. Therefore, it should have the potential as an effective modifier in therapy of leukemia and/or as an immunopotentiator.<br /> (2010 Elsevier Inc. All rights reserved.)
- Subjects :
- B-Lymphocytes drug effects
Cell Line, Tumor
Drug Synergism
Gene Expression drug effects
Humans
Leukemia metabolism
Phagocytes drug effects
Adjuvants, Immunologic pharmacology
Antineoplastic Agents pharmacology
Curcumin pharmacology
NADPH Oxidases metabolism
Phosphoproteins metabolism
Superoxides metabolism
Tretinoin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 395
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 20346917
- Full Text :
- https://doi.org/10.1016/j.bbrc.2010.03.136