Back to Search
Start Over
Antioxidant properties of breast milk in a novel in vitro digestion/enterocyte model.
- Source :
-
Journal of pediatric gastroenterology and nutrition [J Pediatr Gastroenterol Nutr] 2010 Jun; Vol. 50 (6), pp. 670-6. - Publication Year :
- 2010
-
Abstract
- Objectives: There is good evidence to suggest that human breast milk has antioxidant properties. Our primary goal was to investigate the antioxidant properties of human milk in a combined in vitro digestion/cell culture model that more closely replicates conditions in the gastrointestinal system of the preterm infant.<br />Materials and Methods: An in vitro digestion model was developed that incorporates both gastric and intestinal phases, based on reported luminal pH, digestive enzyme levels, and transit times observed in preterm infants. To mimic the human intestinal mucosa, 2 cell lines--Caco-2BBE and HT29-MTX--were cocultured on Matrigel, an artificial basement membrane substrate. Intracellular oxidative stress was measured with 2 broadly selective oxidant-sensitive dyes, and oxidative DNA damage was assessed by means of single-cell gel electrophoresis.<br />Results: Enterocyte differentiation and mucin secretion were observed by 14 seeding of cultures. Direct exposure to digested milk resulted in a loss of transepithelial electrical resistance; however, exogenous mucin mitigated this loss. Data suggested that both milk and digested milk alleviated oxidative stress in the coculture, and both reduced hydrogen peroxide-induced oxidative DNA damage, as demonstrated by the comet assay.<br />Conclusions: Our results support the hypothesis that breast milk reduces oxidative stress in a cell culture model representative of the intestinal mucosa, and also confirmed the suitability of this combined in vitro digestion/cell culture system for investigating the physiologic effects of enteral nutrients such as breast milk, under conditions similar to those existing in the gastrointestinal system of the preterm infant.
- Subjects :
- Caco-2 Cells
Cell Culture Techniques
Cell Differentiation
Collagen
Comet Assay
DNA Damage
Drug Combinations
Electric Impedance
Electrophoresis
Humans
Hydrogen Peroxide
Infant
Intestinal Mucosa physiology
Laminin
Models, Biological
Proteoglycans
Antioxidants pharmacology
Digestion physiology
Enterocytes metabolism
Intestinal Mucosa metabolism
Milk, Human metabolism
Mucins metabolism
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4801
- Volume :
- 50
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of pediatric gastroenterology and nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 20386324
- Full Text :
- https://doi.org/10.1097/MPG.0b013e3181bab2d3