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Proteomic and functional analysis of the noncanonical poly(A) polymerase Cid14.
- Source :
-
RNA (New York, N.Y.) [RNA] 2010 Jun; Vol. 16 (6), pp. 1124-9. Date of Electronic Publication: 2010 Apr 19. - Publication Year :
- 2010
-
Abstract
- The fission yeast Cid14 protein belongs to a family of noncanonical poly(A) polymerases which have been implicated in a broad range of biological functions. Here we describe an extensive Cid14 protein-protein interaction network and its biochemical dissection. Cid14 most stably interacts with the zinc-knuckle protein Air1 to form the Cid14-Air1 complex (CAC). Providing a link to ribosomal RNA processing, Cid14 sediments with 60S ribosomal subunits and copurifies with 60S assembly factors. In contrast, no physical link to chromatin has been identified, although gene expression profiling revealed that efficient silencing of a few heterochromatic genes depends on Cid14 and/or Air1.
- Subjects :
- Chromatin genetics
Chromatin metabolism
Cycloheximide metabolism
Gene Expression Regulation, Fungal
Kinetics
Molecular Weight
Polynucleotide Adenylyltransferase metabolism
Polyribosomes metabolism
Puromycin metabolism
RNA, Fungal genetics
RNA, Fungal metabolism
RNA, Ribosomal genetics
RNA, Ribosomal metabolism
Schizosaccharomyces enzymology
Schizosaccharomyces pombe Proteins metabolism
Polynucleotide Adenylyltransferase chemistry
Schizosaccharomyces pombe Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1469-9001
- Volume :
- 16
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- RNA (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 20403971
- Full Text :
- https://doi.org/10.1261/rna.2053710