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Matrix metalloproteinase (MMP)-13 regulates mammary tumor-induced osteolysis by activating MMP9 and transforming growth factor-beta signaling at the tumor-bone interface.
- Source :
-
Cancer research [Cancer Res] 2010 May 01; Vol. 70 (9), pp. 3494-504. Date of Electronic Publication: 2010 Apr 20. - Publication Year :
- 2010
-
Abstract
- The tropism of breast cancer cells for bone and their tendency to induce an osteolytic phenotype are a result of interactions between breast cancer cells and stromal cells and are of paramount importance for bone metastasis. However, the underlying molecular mechanisms remain poorly understood. We hypothesize that tumor-stromal interaction alters gene expression in malignant tumor cells and stromal cells creating a unique expression signature that promotes osteolytic breast cancer bone metastasis and that inhibition of such interactions can be developed as targeted therapeutics. Microarray analysis was performed to investigate gene expression profiling at the tumor-bone (TB) interface versus the tumor alone area from syngenic mice injected with three different syngenic mammary tumor cell lines that differ in their metastatic potential. We identified matrix metalloproteinase 13 (MMP13), receptor activator of NF-kappaB ligand (RANKL), and integrins binding sialoprotein to be genes upregulated at the TB interface and validated. To determine the functional role of MMP13 in tumor-induced osteolysis, mice with Cl66 mammary tumors were treated with MMP13 antisense oligonucleotides (MMP13-ASO) or control scrambled oligonucleotides (control-ASO). Knockdown of MMP13 expression at the TB interface leads to significant reduction in bone destruction and in the number of activated osteoclasts at the TB interface. Further analysis to evaluate the mechanism of MMP13-dependent osteolytic bone metastasis revealed that MMP13-ASO treatment decreased active MMP9, RANKL levels, and transforming growth factor-beta signaling at the TB interface. Together, our data indicate that upregulation of MMP13 at the TB interface is important in tumor-induced osteolysis and suggest that MMP13 is a potential therapeutic target for breast cancer bone metastasis.<br /> ((c)2010 AACR.)
- Subjects :
- Adenocarcinoma enzymology
Adenocarcinoma pathology
Animals
Bone Neoplasms enzymology
Bone Neoplasms metabolism
Bone and Bones enzymology
Bone and Bones metabolism
Bone and Bones pathology
Cell Line, Tumor
Enzyme Activation
Female
Gene Expression Profiling
Mammary Neoplasms, Experimental metabolism
Matrix Metalloproteinase 13 biosynthesis
Matrix Metalloproteinase 13 genetics
Matrix Metalloproteinase 9 biosynthesis
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase Inhibitors
Mice
Mice, Inbred BALB C
Osteoclasts enzymology
Osteoclasts pathology
Osteolysis genetics
Osteolysis metabolism
Osteolysis pathology
Osteoprotegerin biosynthesis
RANK Ligand biosynthesis
RNA, Messenger biosynthesis
RNA, Messenger genetics
Signal Transduction
Up-Regulation
Bone Neoplasms secondary
Mammary Neoplasms, Experimental enzymology
Mammary Neoplasms, Experimental pathology
Matrix Metalloproteinase 13 metabolism
Matrix Metalloproteinase 9 metabolism
Osteolysis enzymology
Transforming Growth Factor beta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7445
- Volume :
- 70
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 20406980
- Full Text :
- https://doi.org/10.1158/0008-5472.CAN-09-3251