Targeted gene deletions in C. elegans using transposon excision.

Authors :: Frøkjaer-Jensen C
Davis MW
Hollopeter G
Taylor J
Harris TW
Nix P
Lofgren R
Prestgard-Duke M
Bastiani M
Moerman DG
Jorgensen EM
Source :: Nature methods [Nat Methods] 2010 Jun; Vol. 7 (6), pp. 451-3. Date of Electronic Publication: 2010 Apr 25.
Publication Year :: 2010
Abstract: We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.