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[Effect of [Gly14]-Humanin on Abeta(25-35)-induced PC12 cell apoptosis].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2010 May; Vol. 26 (5), pp. 427-30. - Publication Year :
- 2010
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Abstract
- Aim: To investigate the effect of [Gly14]-Humanin overexpression on Abeta(25-35);-induced PC12 cell apoptosis.<br />Methods: Recombinant plasmid pcDNA3.1(-)/HNG-FLAG was transfected into PC12 cells by liposome method. The subclone cell lines were obtained by persistent G418 selection. The HNG gene expression of PC12 cells was detected by immunocytochemistry. After being treated with 25 micromol/L Abeta(25-35); for 24 h, cell viability was determined by MTT assay, and apoptosis rate was detected by using flow cytometric analysis. Hochest33258 staining was used to observe the morphological changes of cellular nuclei.<br />Results: PC12 cell lines stably expressing HNG gene was successfully selected. After being treated with 25 micromol/L Abeta(25-35); for 24 h, cell viability of PC12 cells overexpression HNG was significant elevated compared with empty plasmid transfected cells (P<0.05), and the apoptosis rate was lower significantly (P<0.05). By Hoechst 33258 staining, the nuclei of empty plasmid transfected PC12 cells exhibited highly condensed and fragmented nuclei morphology which was the typical characteristics of apoptosis, and the nuclei of PC12 cells overexpression HNG were round and homogeneously stained.<br />Conclusion: Overexpression of HNG prevented the cell apoptosis induced by Abeta(25-35); in PC12 cells.
- Subjects :
- Animals
Apoptosis genetics
Genetic Vectors genetics
Glycine genetics
Intracellular Signaling Peptides and Proteins chemistry
Intracellular Signaling Peptides and Proteins genetics
PC12 Cells
Rats
Transfection
Amino Acid Substitution
Amyloid beta-Peptides pharmacology
Apoptosis drug effects
Glycine metabolism
Intracellular Signaling Peptides and Proteins metabolism
Peptide Fragments pharmacology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20423647