[Comparative localization of chicken five functional genes and three microsatellites on quail mitotic chromosomes by two-color fish-hybridization].

In order to localize chicken genes and microsatellites we used two-color FISH and chicken chromosome specific BAC-clones. All BAC-clones were verified by PCR. Analysis of the results obtained showed that: maf gene formed one linkage group with mc1r gene (CJA11), aldhlal--with igvps gene (CJA15), pno--with acaca gene (CJA19), fzf--with bmp7 gene (CJA20), cw01--with ubapw2omega gene (CJAW). Microsatellite ADL0254 was localized jointly with insr gene (CJA28), while LE10342 and MCW0330 microsatellites--with hspa5 gene (CJA17). The same work was fulfilled on chicken mitotic chromosomes. We obtained other results. maf gene was localized independently of mc1r (GGA11), aldh1a1 was localized independently of igvps gene (GGA15), and pno gene (GGA19) was localized independently of acaca gene. ADL0254 and LE10342 microsatellites had two sites of localization (GGA28, GGA17 accordingly and other site). Localization for genes cw01 and fzf and for MCW0330 microsatellite was confirmed.